Abstract:
Phytochemicals in Rosmarinus officinalis leaves, their total phenolic content, antioxidant potential
and antiproliferative activity against human prostate (DU145), colon (CT26) and cervical (HeLa 229)
cancer cells were investigated. Extraction was done separately using hexane, dichloromethane, ethyl
acetate and methanol. A total of 32 compounds were identified, eight of which were reported for the
first time. The highest phenolic content was 476.80 ± 0.69 μg/ml for the methanolic extract which
also had the highest antioxidant activity with a minimum inhibitory concentration of 5.39 ± 0.09
mg/ml. Extracts exhibited the highest toxicity against prostate cancer cells and the least against
cervical cancer cells.
