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Introduction: Cryptosporidiosis is a gastrointestinal illness characterized by watery diarrhea and caused by
protozoan parasites belonging to Cryptosporidium species. It occurs in both humans and animals. In immuno competent individuals the infection may resolve without intervention but in immuno-compromised
individuals, it may persist to fatality.
Objectives: To determine the prevalence of cryptosporidiosis, cryptosporidium genotypes and associated
factors among HIV patients.
Methods: This was a cross-sectional study, a total of 1794 stool samples were screened for Cryptosporidium
oocysts, using modified ZN staining and parasite genotypes and sub-genotypes determined using PCR-RFLP
analysis of the 18s RNA subunit and CP47 gene sequencing respectfully. Data was analyzed for measures of
central tendency and statistical associations.
Results: The prevalence of cryptosporidiosis was 3.2% (58/1794) with no significant sex-related differences in
risk of infection with Cryptosporidium (OR=1.2130, 95% CI=0.6895-1.341, P=0.5023). C. hominis was the most
common genotype followed by C. parvum and then C. meleagridis. About 1% of the samples had a mixed
infection of C. hominis and C. parvum. Cryptosporidiosis significantly associated with diarrhea (OR= 4.7087,
P≤0.0001), headache (OR=2.218, P= 0.0033), and skin rash (OR=2.2179, P=0.0279).
Conclusion: The prevalence of cryptosporidiosis among HIV positive was of 3.2% and within the range of 3-
4% reported in other parts of Kenya and within the East African region. Diarrhea, headache, low CD4
counts and skin rash were significantly associated with Cryptosporidium infection. |
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