Please use this identifier to cite or link to this item:
http://ir.mu.ac.ke:8080/jspui/handle/123456789/5582
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wabwoba, Byrum W. | - |
dc.contributor.author | Anjili, Christopher O. | - |
dc.contributor.author | Ngeiywa, Moses M. | - |
dc.contributor.author | Ngure, Peter K. | - |
dc.contributor.author | Kigondu, Elizabeth M. | - |
dc.contributor.author | Ingonga, Johnstone I | - |
dc.contributor.author | Makwali, Judith | - |
dc.date.accessioned | 2021-12-08T08:20:24Z | - |
dc.date.available | 2021-12-08T08:20:24Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | http://ir.mu.ac.ke:8080/jspui/handle/123456789/5582 | - |
dc.description.abstract | Background & objectives: Several plant products have been tested and found to possess anti- leishmanial activity. The present study was undertaken to establish whether methanolic extract of Allium sativum Linn has antileishmanial activity in comparison to standard drugs. Methods: Methanolic extract of A. sativum bulbs was screened for in vitro and in vivo antileishmanial activity against Leishmania major strain (NLB 145) and L. donovani strain (NLB 065). Pentostam® and Amphotericin B® were used as standard drugs. BALB/c mice and golden hamsters (Mesocricetus auratus) were used in in vivo studies on L. major and L. donovani respectively. Results: The extract exhibited very low cytotoxicity (IC50 >450 μg/ml) against Vero cells. The extract had significantly better (p <0.001) leishmanicidal activity against both species (IC50 34.22 μg/ml to L. major, 37.41 μg/ml to L. donovani) than Pentostam. However, the activity was significantly lower (p <0.001) than that of Amphotericin B against both the species. At a concentration of 250 μg/ml, the extract induced the production of 60 μM of nitric oxide, a ten-fold up-regulation in activated macrophages. The multiplication indices for L. major amastigotes treated in 100 μg/ml were significantly different (p <0.05). Treatment with the extract, daily for 28 days led to a significant reduction (p <0.05) in footpad swelling in BALB/c mice; similar activity noticed in the treatment with standard drugs. The Leishman-Donovan Units (LDU) for the extract treated animals were significantly higher (p <0.05) than those of standard drugs, but lower compared to the negative control. Interpretation & conclusion: Since the mechanism of action for the methanolic extract is apparently immunomodulatory, garlic compounds could be purified and tried as complementary medicine in the management of leishmaniases. | en_US |
dc.language.iso | en | en_US |
dc.subject | Amastigotes | en_US |
dc.subject | Promastigotes | en_US |
dc.subject | Antimonials | en_US |
dc.subject | Leishmaniasis | en_US |
dc.subject | Methanolic extract | en_US |
dc.title | Experimental chemotherapy with allium sativum (Liliaceae) methanolic extract in rodents infected with leishmania major and leishmania donovani | en_US |
dc.type | Article | en_US |
Appears in Collections: | School of Biological and Physical Sciences |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Judith Mwakali etal.pdf | 187.27 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.